A METHOD FOR BINDING A PROTEIN CONSISTING OF PROTEIN A OR CONSISTING OF AT LEAST ONE DOMAIN OF THE A TO E DOMAINS OF THE PROTEIN A TO THE SUBSTRATE

摘要

The object of the present invention is to provide a method for immobilizing the SpA protein on the surface of a substrate with high density without causing dimerization. The following method solves the object. That is, the method for binding a protein to a surface of a substrate, comprising steps (A) to (B): step (A) of preparing said protein to the surface, step (B) of supplying said protein to the surface, wherein said protein consists of a Protein A or at least one domain of A to E of said Protein A, and said protein comprises C-terminal modified amino acid sequence represented by SEQ ID:1(SFNRSEC).