摘要 |
The present invention relates to a method of preparing mycobacterial CWS from mycobacterial cells through deproteination and delipidation processes using a surfactant. More specifically, the invention relates to a method for preparing mycobacterial CWS, the method comprising the steps of: (A) adding a surfactant to killed mycobacterial cells and heat-treating the cells in the presence of the surfactant, thereby delipidating and deproteinating the cells; and (B) washing the delipidized and deproteinized mycobacterial cells. According to the invention, mycobacterial CWS can be economically mass-produced by a simple process without using enzyme. Also, the mycobacterial CWS prepared according to the invention can be stably stored as a homogenized suspension without forming an aggregate, and thus can be more efficiently used in anticancer immunotherapy or adjuvant therapy. |