发明名称 Method for assaying protein-protein interaction
摘要 The invention relates to a method for determining if a test compound, or a mix of compounds, modulates the interaction between two proteins of interest. The determination is made possible via the use of two recombinant molecules, one of which contains the first protein a cleavage site for a proteolytic molecules, and an activator of a gene. The second recombinant molecule includes the second protein and the proteolytic molecule. If the test compound binds to the first protein, a reaction is initiated whereby the activator is cleaved, and activates a reporter gene.
申请公布号 US8017398(B2) 申请公布日期 2011.09.13
申请号 US20060346759 申请日期 2006.02.02
申请人 LIFE TECHNOLOGIES CORPORATION;THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK 发明人 LEE KEVIN J.;AXEL RICHARD;STRAPPS WALTER;BARNEA GILAD
分类号 C12N15/09;C12N;C12N1/21;C12N15/00;C12N15/85;C12Q1/68;G01N33/53;G01N33/68;G01N33/94 主分类号 C12N15/09
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