RECOMBINANT PROTEIN CONSTRUCT DSD-sp--GAL EXHIBITING ENZYMATIC ACTIVITY OF THERMOSTABLE -GALACTOSIDASE (LACTASE) AND AFFINE CONNECTABLE WITH DEXTRANE, PLASMID DNA pGD-10 DETERMINING DSD-sp--GAL BIOSYNTHESIS, AND Escherichia coli DH5/PGD-10 PRODUCER STRAIN

摘要

FIELD: medicine. ^ SUBSTANCE: there is reconstructed recombinant plasmid DNA pGD-10 coding a chimeric protein construct DsD-sp--GAL containing a dextrane-binding domain DBD from Leuconostoc mesenteroides microorganism which is genetically combined through a glycine-serine spacer with thermostable beta-galactosidase from thermophilic Thermoanaerobacter ethanolicus microorganism. The Escherichia coli DH5/pGD-10 strain producing the recombinant protein construct DsD-sp--GAL is produced by transforming the cells of parental Escherichia coli DH5 strain by recombinant plasmid DNA pGD-10. ^ EFFECT: invention allows producing protein DsD-sp--GAL exhibiting enzymatic activity of thermostable beta-galactosidase and affine connectable with dextrane. ^ 3 cl, 4 dwg