ATTENUATED STRAIN OF MELON YELLOW SPOT VIRUS AND METHOD FOR PRODUCING THE SAME, AND METHOD FOR PREVENTING YELLOW SPOT DISEASE IN CUCURBITACEOUS PLANT BY INOCULATING THE SAME

摘要

PROBLEM TO BE SOLVED: To provide an attenuated strain of melon yellow spot virus (MYSV) and a method for producing the same, as well as a method for preventing yellow spot disease in cucurbitaceous plants by inoculating the attenuated virus strain.

SOLUTION: There are disclosed a method for producing an attenuated strain of MYSV comprising: (a) a process of inoculating MYSV into the leaves of plants except a host plant in nature; (b) a process of treating the MYSV-inoculated plants at a high temperature; and (c) a process of selecting and collecting the attenuated strain of MYSV from the inoculated leaves, and an attenuated strain of MYSV that is obtained by the production method and has RNA encoding a specific amino acid sequence or its complementary strand.

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