发明名称 |
HYDROXYLASE GENE AND USE THEREOF |
摘要 |
A vitamin D 3 hydroxylase is purified from Pseudonocardia autotrophica cell, and a primer is designed based on amino acid sequence obtained from hydroxylase. Subsequently, PCR is conducted using genomic DNA of Pseudonocardia autotrophica as a template to clone a gene for the vitamin D 3 hydroxylase. By conducting a conversion reaction using a microorganism in which the vitamin D 3 hydroxylase gene is expressed using a proper expression system, a hydroxide of vitamin D or the like (e.g., hydroxy vitamin D 3 ) can be produced with high efficiency. |
申请公布号 |
EP2357229(A4) |
申请公布日期 |
2011.08.17 |
申请号 |
EP20070743689 |
申请日期 |
2007.05.18 |
申请人 |
MERCIAN CORPORATION;NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCEAND TECHNOLOGY |
发明人 |
FUJII, YOSHIKAZU;TAMURA, TOMOHIRO |
分类号 |
C12N15/09;C12N1/21;C12N9/04;C12P7/02;C12P33/06 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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