| 摘要 |
PURPOSE: A method for preparing a medium for culturing succinic acid-producing strains is provided to produce a large amount of succinic acid with eco-friendly method. CONSTITUTION: A method for preparing a medium for culturing succinic acid-producing strain comprises a step of determining content ratio of magnesium ion source, calcium ion source, and potassium phosphate, dibasic(K_2HPO_4) using Taguchi method. The magnesium ion source is selected from magnesium hydroxide(Mg(OH)_2), magnesium borate(Mg_2B_2O_5), magnesium sulfate(MgSO_4), magnesium bromide(MgBr_2), magnesium carbonate(MgCO_3), magnesium phosphate(Mg(H_2PO_4)_2), magnesium hexafluorosilicate(MgSiF_6·6H_2O) and magnesium chloride(MgCl_2). The calsium ion source is selected from calcium carbonate(CaCO_3), calcium hydroxide(Ca(OH)_2), calcium chloride(CaCl_2), calcium cyclamate (Ca(C_6H_11NHSO_3)_2), calcium phosphate(Ca_3(PO_4)_2), and calcium stearate(Ca(C_18H_35O_2)_2). The strain is Mannheimia genus.
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