METHODS AND COMPOSITIONS FOR HIGH YIELD, SPECIFIC AMPLIFICATION

摘要

The present invention is directed to methods and compositions for amplifying nucleic acids. Included in the present invention are methods and compositions that amplify nucleic acids with high yield with the formation of unstable target extension products, preferably with minimal or no introduction of allelic bias. Also included in the present invention are high yield, instability primers for use in amplification methods, as multiplexed amplification methods.