| 摘要 |
Disclosed is a method of testing a sample to provide an indication of the level of bacteria and/or number of somatic cells present in the sample, the method including contacting the sample with a reagent which reacts with ATP to produce light emissions, and subsequently, at the beginning of a measurement period, contacting the sample with an extractant in the presence of the reagent, the extractant rupturing cells present in the sample to release cell-bound ATP to react with the reagent to produce light emissions, and immediately after contacting the sample with the extractant, detecting the light emissions with a light detecting device, and the method includes during an initial measurement period immediately after contacting the sample with the extractant, detecting the level of light emissions, and in a subsequent measurement period, detecting the level of light emissions, analysing the detected values to determine a rate of change of light emissions during the subsequent measurement period, due to cell-bound ATP being released and reacting with the reagent, and at least where the thus determined rate of change of light emissions indicates a level of bacteria and/or somatic cells in the sample above a threshold, generating a signal.
|
