发明名称 |
BRANCHED-CHAIN FATTY ACIDS AND BIOLOGICAL PRODUCTION THEREOF |
摘要 |
A method for producing anteiso fatty acid is provided. The method comprises culturing a cell comprising at least one exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes at least one of the following reactions: conversion of pyruvate to citramalate; conversion of citramalate to citraconate; conversion of citraconate to ß-methyl-D-malate; conversion of ß-methyl-D-malate to 2-oxobutanoate; or conversion of threonine to 2-oxobutanoate, under conditions allowing expression of the polynucleotide(s) and production of anteiso fatty acid. Optionally the cell further comprises at least one exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes conversion of 2-oxobutanoate to 2-aceto-2-hydroxy-butyrate, conversion of 2-aceto-2-hydroxy-butyrate to 2,3-dihydroxy-3-methylvalerate, and/or conversion of 2,3-dihydroxy-3-methylvalerate to a-keto-3-methylvalerate. A cell that produces anteiso fatty acid and a method of using the cell to produce anteiso fatty acid also are provided. |
申请公布号 |
CA2781730(A1) |
申请公布日期 |
2011.07.21 |
申请号 |
CA20102781730 |
申请日期 |
2010.12.21 |
申请人 |
THE PROCTER & GAMBLE COMPANY |
发明人 |
SAUNDERS, CHARLES WINSTON;XU, JUN;GREEN, PHILLIP RICHARD;CODY, DAVID BLAIR;KHAMBATTA, ZUBIN SAROSH |
分类号 |
C12N15/70;C12N9/02;C12N9/10;C12N9/88;C12N15/52;C12P7/64 |
主分类号 |
C12N15/70 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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