Mus Musculus ANIMAL HYBRID CULTURED CELL STRAIN PRODUCING benzo[a]pyrene AND benz[a]anthraccene SPECIFIC MONOCLONAL ANTIBODIES

摘要

FIELD: medicine. ^ SUBSTANCE: hybridoma techniques involving the fusion of myeloma of Sp-2/0 mice and lymphocytes and splenocytes of BALB/c mice immunised by a conjugate of benzo[a]pyrene and a bovine serum albumin, with using polyethylene glycol of molecular weight 1500 for cell fusion, followed by limit dilution cloning are used to produced a Mus. Musculus BpBal animal hybrid cultured cell strain deposited in RKKK(P) of the Institute of Cytology of the Russian Academy of Sciences, Registration No. 723 D producing the benzo[a]pyrene and benz[a]anthracene specific monoclonal antibodies. The hybridoma strain produces the monoclonal antibodies mAB showing high Bp and Ba specificity and low cross-responsiveness with non-cancerogenic polycyclic aromatic hydrocarbons (PAH) and with endogenous compounds (aromatic amino acids and steroid hormones). ^ EFFECT: using the BpBal mAB allows producing sensitive and specific test systems for definition cancerogenic PAHs, the related antibodies in human and animal biological fluids, and searching immune mimetic peptides and producing anticancerogenic PAH vaccines. ^ 1 dwg, 3 tbl, 3 ex