发明名称 |
METHODS AND COMPOSITIONS FOR TARGETED POLYNUCLEOTIDE MODIFICATION |
摘要 |
A variety of methods and compostions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism. |
申请公布号 |
CA2793596(A1) |
申请公布日期 |
2011.07.07 |
申请号 |
CA20102793596 |
申请日期 |
2010.12.30 |
申请人 |
PIONEER HI-BRED INTERNATIONAL, INC. |
发明人 |
GORDON-KAMM, WILLIAM J.;LOWE, KEITH S.;PETERSON, DAVID J.;SCELONGE, CHRISTOPHER J.;ST. CLAIR, GRACE M.;WANG, BING-BING |
分类号 |
C12N15/82;A01H5/00;C07K14/415 |
主分类号 |
C12N15/82 |
代理机构 |
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