发明名称 |
EXPRESSION CASSETTE FOR LACTASE DEHYDROGENASE, TRANSFORMED YEAST AND METHOD OF PRODUCING LACTIC ACID |
摘要 |
Provided are a gene expressing cassette coding lactate dehydrogenase that is needed for prevention of deterioration in lactic acid yield and lactic acid production rate in continuous culture with simultaneous filtration of a yeast strain having a lactic acid-producing ability, which achieves high optical purity, high lactic acid yield and high lactic acid production rate simultaneously, a yeast strain having the cassette and a method of producing lactic acid by culturing the yeast strain. The lactate dehydrogenase-expressing cassette according to the present invention is a lactate dehydrogenase-expressing cassette, comprising a gene coding lactate dehydrogenase connected to a site downstream of a promoter, the promoter being a promoter of a gene showing a gene expression amount larger by 5 times or more than the average relative expression amount of all genes after 50 hours from start of culture in continuous culture with simultaneous filtration of a yeast strain having a lactic acid-producing ability. |
申请公布号 |
EP2221373(A4) |
申请公布日期 |
2011.07.06 |
申请号 |
EP20080856191 |
申请日期 |
2008.12.05 |
申请人 |
TORAY INDUSTRIES, INC. |
发明人 |
SAWAI, KENJI;SAWAI, HIDEKI |
分类号 |
C12N15/09;C12N1/19;C12P7/56 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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