发明名称 |
POLYPEPTIDE MODIFICATION METHOD FOR PURIFYING POLYPEPTIDE MULTIMERS |
摘要 |
Disclosed is a method for purifying or producing, by modifying the binding strength to Protein A, a multispecific antibody having binding activity for at least two kinds of antigens, wherein said multispecific antibody is purified or produced at a high purity and efficiency with only a Protein A purification step. The disclosed method for purifying or producing a multispecific antibody is characterized by modifying the amino acid residue of the antibody heavy chain constant regions and/or the antibody heavy chain variable regions. By introducing such amino acid modifications into the antibody, a multispecific antibody which has a modified binding strength to Protein A and has blood plasma retentivity greater than or equal to that of human IgG1 can be acquired at a high purity and efficiency. |
申请公布号 |
WO2011078332(A1) |
申请公布日期 |
2011.06.30 |
申请号 |
WO2010JP73361 |
申请日期 |
2010.12.24 |
申请人 |
CHUGAI SEIYAKU KABUSHIKI KAISHA;IGAWA, TOMOYUKI;SAMPEI, ZENJIRO;WAKABAYASHI, TETSUYA;ITO, ERIKO |
发明人 |
IGAWA, TOMOYUKI;SAMPEI, ZENJIRO;WAKABAYASHI, TETSUYA;ITO, ERIKO |
分类号 |
C12N15/00;A61K39/395;C07K1/22;C07K16/46;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N15/09 |
主分类号 |
C12N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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