| 摘要 |
Disclosed is a method for freezing sorted sperm cells compromised by a sorting event comprising the steps of: subjecting a plurality of sperm cells to sorting stresses; selecting said plurality ofsperm cells for a desired characteristic; adding a protein-containing sperm cell extender to said plurality of selected sperm cells to form a first extended sperm cell mixture; cooling said first extended sperm cell mixture to create a first cooled extended sperm cell mixture; adding a protein-free cryoprotectant-containing sperm cell extender to said first cooled extended sperm cell mixture to form a second cooled extended sperm cell mixture; and freezing said second cooled extended sperm cell mixture.
|
