Method for detection of microorganism comprising treating a sample with an enzyme which decomposes non microorganism cell followed by treatment with a topoisomerase poison and/or a DNA gyrase poison

摘要

Disclosed is a method for preparing a measurement sample for detecting live cells of a microorganism in a test sample by flow cytometry, which comprises the following steps: a) the step of treating the test sample with an enzyme selected from lipolytic enzymes and proteases which having an activity of decomposing other than those of the microorganism, and colloidal particles of proteins or lipids existing in the test sample, and b) the step of treating the test sample with a topoisomerase poison selected from the group consisting of amsacrine, camptothecin, doxorubicin, ellipticine, etoposide, mitoxantrone, saintopin, topotecan, CP-115,953 and ethidium monoazide and/or a DNA gyrase poison selected from the group consisting of ciprofloxacin, ofloxacin, enoxacin, pefloxacin, fleroxacin, norfloxacin, nalidexic acid, oxolinic acid and piromidic acid. Further disclosed are methods for detecting live, injured and dead cell; and kits for preparing a measurement thereof.