| 摘要 |
The invention relates to immune-enzyme analysis of brassinosteroid phytohormones, namely to a conjugate of 28-norbrassinolide with bovine serum albumin which may be used as immunogen for producing an antiserum specific for 7-oxa-6-oxobrassinosteroids. The task of the present invention is 28-norbrassinolide-bovine serum albumin conjugate for producing an antiserum specific for 7-oxa-6-oxobrassinosteroids. The problem is solved by a conjugate of 28-norbrassinolide with bovine serum albumin which was prepared by reaction of Noxysuccinimideester of (22R,23R)-2α,3α,22,23-tetrahydroxy-26-(hemisuccinat)-B-homo-7-oxa-5α-cholestan-6-one (hapten) with bovine serum albumin in dioxane (pH 8.5) followed by dialysis purification and lyophilization of conjugate. |
| 申请人 |
THE STATE SCIENTIFIC INSTITUTION "THE INSTITUTE OF BIOORGANIC CHEMISTRY, THE NATIONAL ACADEMY OF SCIENCE OF BELARUS" |
发明人 |
KHRIPACH VLADIMIR, ALEXANDROVICH;ZHABINSKIJ VLADIMIR, NIKOLAEVICH;ANTONCHIK ALEXEJ, VLADIMIROVICH;LITVINOVSKAYA RAISA, PETROVNA;DRACH SVETLANA, VASILIEVNA;SVIRIDOV OLEG, VASILIEVICH;PRYADKO ANDREJ, GEORGIEVICH;NOVIK TATIANA, VALENTINOVNA;MATVEENTSEV VITALIJ, DEMIDOVICH |
