发明名称 METHOD FOR QUALITATIVE AND QUANTITATIVE DETECTION OF COMMON WHEAT
摘要 PROBLEM TO BE SOLVED: To provide a method for qualitative and/or quantitative detection with high specificity and sensitivity of common wheat among wheat contained in various test samples, such as food raw material and processed food. SOLUTION: The method comprises: a PCR amplification using two primers, the one consisting of a specific base sequence and the other consisting of a specific base sequence that is different from that of the former primer, and the nucleic acid extracted from a test sample as the template; and the detection of the presence of PCR amplified product. The kit for the method is also provided. COPYRIGHT: (C)2011,JPO&INPIT
申请公布号 JP2011125309(A) 申请公布日期 2011.06.30
申请号 JP20090289340 申请日期 2009.12.21
申请人 NATIONAL AGRICULTURE & FOOD RESEARCH ORGANIZATION;NIPPON FLOUR MILLS CO LTD;NISSHIN SEIFUN GROUP INC 发明人 KITSUTA KAZUMI;FURUI SATOSHI;MANO JUNICHI;MATSUOKA YASUYUKI;ARAMI SHINICHIRO;SATO EMI;HARAGUCHI HIROYUKI;KURIMOTO YOICHI;IMAI SHINJIRO;TANAKA KEIKO
分类号 C12Q1/68;C12N15/09 主分类号 C12Q1/68
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