发明名称 |
Mutations caused by activation-induced cytidine deaminase |
摘要 |
Methods for causing mutations in genes expressed in eukaryotic cells are provided. The methods involve expressing an activation-induced cytidine deaminase (AID) in the cells. The mutated genes can be any gene that is operably linked to a promoter, where the gene is within about 2 kilobases of the promoter. Examples include antibody genes. Also provided are cells expressing AID. The cells can be from any eukaryote, and include hybridoma cells and myeloma fusion partners.
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申请公布号 |
US2011143440(A1) |
申请公布日期 |
2011.06.16 |
申请号 |
US20100804089 |
申请日期 |
2010.07.13 |
申请人 |
MARTIN ALBERTO;SCHARFF MATTHEW D |
发明人 |
MARTIN ALBERTO;SCHARFF MATTHEW D. |
分类号 |
C12N15/79;C12N5/10;C12N5/12;C12N5/22;C12N5/26;C12N15/10;C12N15/63;C12N15/85;C12P21/02;C12Q1/02;C12Q1/68 |
主分类号 |
C12N15/79 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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