Method of nucleic acid sequencing

摘要

The present invention relates to a method for sequencing a nucleic acid molecule comprising:
a. using a first colony to provide a plurality of single stranded nucleic acid molecules having the same sequence and hybridized to primers in a manner to allow primer extension;
b. using a second colony to provide a plurality of single stranded nucleic acid molecules having the same sequence and hybridized to primers in a manner to allow primer extension;
c. providing each colony with a nucleic acid polymerase and a labeled nucleotide under conditions allowing extension of the primers if a complementary base or plurality of bases is present at the appropriate position in the single stranded nucleic acid molecules in the colonies;
d. detecting whether or not the labeled nucleotide has been incorporated into extended primers; and
e. repeating steps c. and d. one or more times so that extended primers comprising a plurality of labels are provided.