METHOD OF PREPARING CELLS SUSCEPTIBLE TO TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHY AND PERSISTENT TSE - INFECTED CELL

摘要

PURPOSE: A method for preparing TSE(transmissible spongiform encephalopathy) sensitive cells is provided to develop novel drugs. CONSTITUTION: A method for preparing TSE-sensitive cells comprises: a step of isolating normal prion protein gene from the brain of an animal which infected by TSE and cloning; a step of inserting the gene to a lentivirus transfer vector to prepare infectious recombinant lentivirus; and a step of inoculating the infectious recombinant lentivirus to non-animal mammal cell line to prepare transformed cells which express a normal prion protein.