发明名称 |
JFH-1 BASED HCV CELL CULTURE SYSTEMS FOR NS5A OF GENOTYPES 1-7 |
摘要 |
The present inventors developed hepatitis C virus recombinants expressing NS5A from genotype Ia, 1b, 2a, 3a, 4a, 5a, 6a or 7a in the context of a genotype 2a backbone. Additional recombinants express NS5A and the structural proteins (Core, E1 and E2), p7 and NS2 from genotype 1a, 1b, 3a, 4a, 5a, 6a or 7a in the genotype 2a backbone. Sequence analysis of the recombinants recovered after viral passage in Huh7.5 cells revealed adaptive mutations in NS5A and/or NS3. The importance of these mutations for improved growth kinetics was shown in reverse genetic studies. |
申请公布号 |
WO2011039639(A3) |
申请公布日期 |
2011.05.26 |
申请号 |
WO2010IB02620 |
申请日期 |
2010.10.01 |
申请人 |
HVIDOVRE HOSPITAL;KOBENHAVNS UNIVERSITET;SCHEEL, TROELS KASPER, HOYER;GOTTWEIN, JUDITH, M.;JENSEN, TANJA, BERTELSEN;BUKH, JENS |
发明人 |
SCHEEL, TROELS KASPER, HOYER;GOTTWEIN, JUDITH, M.;JENSEN, TANJA, BERTELSEN;BUKH, JENS |
分类号 |
C12N7/00;A61K39/29;C07K14/18;C12N7/02;G01N33/50 |
主分类号 |
C12N7/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|