NUCLEIC ACID PROBE FOR ASSAYING NUCLEIC ACID

摘要

PROBLEM TO BE SOLVED: To provide a new mixture for assaying nucleic acid characterized by enabling a nucleic acid assay to (1) require no step of diluting the target nucleic acid, and (2) require no procedure of changing a probe concentration depending on the concentration of the target nucleic acid.

SOLUTION: There are disclosed (1) a mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye, (2) a mixture for measuring the Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye, (3) a mixture which comprises one internal standard nucleic acid and a double nucleic acid probe labeled with two fluorescent dyes, and a method for assaying a nucleic acid by making use the mixture.

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