发明名称 |
Method for Amplifying Target Nucleic Acid Sequence, Method for Detecting Mutation Using the Same, and Reagent Used for the Same |
摘要 |
The present invention provides a method for detecting a mutation capable of detecting a mutation with high sensitivity and high reliability in one reaction system. Using primers (Xmt) and (Xwt), a target nucleic acid sequence whose objective base to be detected is a mutant-type is amplified with amplification efficiency higher than a target nucleic acid sequence whose objective base to be detected is a normal-type. The (Xmt) is a primer that is complementary to a region including a mutant-type base in the template nucleic acid and has a base complementary to a mutant-type base at a 3′ region, and the (Xwt) is a primer that is complementary to a region including a normal-type base in the template nucleic acid and has a base complementary to a normal-type base at a 3′ region. It is preferable that amplification efficiency by the (Xmt) with reference to a mutant-type template nucleic acid is higher than that by the (Xwt) with reference to a normal-type template nucleic acid. Then, a signal value that shows a molten state of a hybridization product between the thus obtained amplification product and the probe is measured, and the presence or absence of the mutation of the objective base site is determined from a change in the signal value accompanying a change in the temperature.
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申请公布号 |
US2011117568(A1) |
申请公布日期 |
2011.05.19 |
申请号 |
US200913002194 |
申请日期 |
2009.07.02 |
申请人 |
ARKRAY, INC |
发明人 |
HIRAI MITSUHARU;HOSOMI TOSHIYA;IGUCHI AKI |
分类号 |
C12Q1/68;C07H21/04;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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地址 |
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