ELECTROADDRESSING AND IN-FILM BIOPROCESSING USING STIMULI-RESPONSIVE HYDROGEL-FORMING POLYMERS
摘要
Methods for the generation of hydrogels formed by electrodeposition of an electroaddressable polymer are described. The hydrogels may contain one or more cell populations electroaddressed or electroaddressable to a location within the hydrogel and where the cells of the cell populations are entrapped by the hydrogel and are capable of expansion within the hydrogel and may be releasable from the hydrogel. Further provided are electroaddressable polysaccharide blends for the in-film expansion of a cell population, allowing probing of the cells and formation of immunocomplexes. Further provided are methods of using hydrogels containing electroaddressed or electroaddressable cell populations in in-film bioprocessing methods such as cell-based biosensing, protein-based biosensing, and in studies of cell signaling.
申请公布号
WO2010129279(A3)
申请公布日期
2011.05.05
申请号
WO2010US32575
申请日期
2010.04.27
申请人
UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE;SHI, XIAO-WEN;LIU, YI;PAYNE, GREGORY, F.;YANG, XIAOHUA