| 摘要 |
<p>A method for detecting a target analyte associated with nano-sized gold- and /or silver- containing detecting labels in a microarray of samples, wherein the labels indicate presence or absence of a target analyte in a sample, comprises (i) sequentially illuminating at least two sample groups with at least two different monochromatic lights, said sample groups including (a) a first sample group containing at least one sample potentially containing the target analyte, and (b) a second sample group serving as positive control or negative control, (ii) detecting intensity of light reflected, absorbed or emitted from each of said sample groups when illuminated with each of the monochromatic lights, (iii) recording groups of values associated with the light intensity reflected, absorbed or emitted from the sample groups, with each group of values associated with each of the monochromatic lights for each of the sample groups, (iv) comparing the groups of values associated with said sample groups and each of said monochromatic lights; and (v) determining the presence or absence of the target analyte based on the comparison in step (iv), wherein said monochromatic lights include at least a blue monochromatic light or a monochromatic light with a frequency in the range of 10nm to 100nm. Apparatus for performing such method is also disclosed.</p> |
