发明名称 |
Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy |
摘要 |
Methods are provided for the synthesis and secretion of recombinant proteins preferably large mammalian proteins or hetero-multimeric proteins at high levels and for prolonged time in polyploid, preferably diploid yeast. These methods use various mating competent yeast, including Pichia. In a preferred embodiment, a first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.
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申请公布号 |
US7927863(B2) |
申请公布日期 |
2011.04.19 |
申请号 |
US20060429053 |
申请日期 |
2006.05.08 |
申请人 |
ALDER BIOPHARMACEUTICALS, INC.;KECK GRADUATE INSTITUTE |
发明人 |
CREGG JAMES M.;LATHAM JOHN;LITTON MARK;SCHATZMAN RANDALL;TOLSTORUKOV ILYA I. |
分类号 |
C12N1/00;C07K16/00;C07K16/28;C12N1/19;C12N15/74;C12P21/04;C12Q1/68 |
主分类号 |
C12N1/00 |
代理机构 |
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地址 |
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