| 摘要 |
Described is a method for the delivery of a bio-active molecule to a mammal comprising the steps of; (i) administrating a temperature sensitive carrier comprising a bio-active molecule; (ii) using a first ultrasound pulse so as to release the bio-active molecule from the temperature sensitive carrier, and (iii) using a second ultrasound pulse so as to facilitate sonoporation and subsequent intracellular uptake of the bio-active molecule, wherein step (iii) can be performed before, and/or after steps (i) to (ii). The method according to the invention is applicable to a broad range of bio-active molecules such as nucleotide based drugs (e.g. siRNA, mRNA, antisense, plasmid DNA) or small molecules that otherwise would not cross the cell membrane, including peptides and small proteins.
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