发明名称 |
Process for isolating vascular endothelial cells from embryoid bodies differentiated from embryonc stem cells |
摘要 |
The present invention provides a process for isolating vascular endothelial cells from embryoid bodies differentiated from embryonic stem cells, which comprises: (a) treating embryoid bodies differentiated from embryonic stem cells with 0.005-0.015% trypsin and 0.05-0.15 mM ethylenediaminetetraacetate (EDTA) to obtain vascular endothelial cell clusters; and (b) treating the vascular endothelial cell clusters with 0.1-0.5% trypsin and 0.5-2 mM EDTA so as to separate the vascular endothelial cell clusters into single cells.
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申请公布号 |
US7919318(B2) |
申请公布日期 |
2011.04.05 |
申请号 |
US20070913295 |
申请日期 |
2007.06.13 |
申请人 |
CHABIO & DIOSTECH CO., LTD.;COLLEGE OF MEDICINE POCHON CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION |
发明人 |
CHUNG HYUNG-MIN;MOON SUNG-HWAN;KIM JU-MI;LEE SOO-HONG |
分类号 |
C12N5/02;C12N5/071 |
主分类号 |
C12N5/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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