| 摘要 |
FIELD: medicine, veterinary science. ^ SUBSTANCE: invention refers to veterinary science, particularly to method for producing drugs. Substance of the method consists in the fact that a preparation is made of drone larvae aged 5 to 14 days which are placed in a freezing chamber at temperature (-4C-(-5C)) for 12-14 h. Further, a brood is defrozen to temperature +18C and homogenised for 3-5 minutes. It is added with diacetophenonylselenide 3.5-4 mg per 100 ml of a homogenate, then added with acetylsalicylic acid in amount 0.2 % to the volume, mixed thoroughly for 2-3 min to produce a homogeneous mass, poured in sterile flasks 100 ml, closed with sterile bulky plugs and placed in a thermostat at temperature +50-(+ 55C) for 35-40 min, at the end of a preset time, the flasks are moved to another thermostat for incubation at +37C for 8 h. A cycle is repeated for three times. Then, it is cooled to room temperature. In sterile conditions, mixed homogenate and diacetophenonylselenide is added with a sterile drying medium (saccharose-gelatin mixture containing 10% of saccharose and 5% of gelatin) in the homogenate to drying medium ratio 1:10. The mixture is mixed for 2-3 minutes, and filtered in a sterile box through 4-5 layers of a sterile gauze, frozen at temperature -50C and sublimated in a vacuum chamber at shelve preheat temperature +30-(+45C), when achieving preparation temperature +20C-(+22C), shelve temperature is decreased to +25C and dried up for 22-24 h. ^ EFFECT: method allows producing an effective preparation using which allows higher total and specific resistance of animal organism. ^ 5 ex, 2 tbl, 1 dwg |
