METHODS OF DETECTING LONG RANGE CHROMOSOMAL INTERACTIONS
摘要
The present invention relates to a method of monitoring epigenetic changes comprising monitoring changes in conditional long range chromosomal interactions at at least one chromosomal locus where the spectrum of long range interaction is associated with a specific physiological condition, the method comprising the steps of:—(i) in vitro crosslinking of said long range chromosomal interactions present at the at least one chromosomal locus; (ii) isolating the cross linked DNA from said chromosomal locus; (iii) subjecting said cross linked DNA to restriction digestion with an enzyme that cuts at least once within the at least one chromosomal locus; (iv) ligating said cross linked cleaved DNA ends to form DNA loops; (v) identifying the presence of said DNA loops; wherein the presence of DNA loops indicates the presence of a specific long range chromosomal interaction.
申请公布号
EP2297340(A1)
申请公布日期
2011.03.23
申请号
EP20090757758
申请日期
2009.06.02
申请人
OXFORD BIODYNAMICS LIMITED
发明人
RAMADASS, AROUL, SELVAM;AKOULITCHEV, ALEXANDRE;MELLOR, ELIZABETH, JANE