发明名称 |
Kits for displacement Sandwich Immuno-PCR |
摘要 |
<p>The present invention is directed to labeling antibodies with DNA in a convenient kit format for the purpose of producing and utilizing nucleic acid detection immunoassays. The process comprises treating a reporter antibody with a chemicaly activated strand of DNA, which serves as a template for any DNA amplification system. The reporter antibody-DNA conjugate is used to form a complex with corresponding antigen. The complex can be detected by the production of multiple copies of the DNA template. The kit may also provide an activated DNA sequence to label a capture antibody. The DNA conjugated to the capture antibody is modified to contain a high affinity binding ligand for the purpose of coating a solid support. The coated solid support functions to capture the immune complex containing reporter antibody in a sandwich assay format.; The DNA sequence of the capture antibody may also have properties providing for the detachment of the complex from the solid support.</p> |
申请公布号 |
EP2290100(A2) |
申请公布日期 |
2011.03.02 |
申请号 |
EP20100184768 |
申请日期 |
2003.11.03 |
申请人 |
IRIS MOLECULAR DIAGNOSTICS, INC. |
发明人 |
JABLONSKI, ED;DRIVER, DAVID;ADAMS, TOM |
分类号 |
C12Q1/68;C12N;G01N33/53;G01N33/536;G01N33/537;G01N33/543 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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