SELECTIVE MARKER FOR TARGET BIOPOLYMER

摘要

A selective fluorescent marker for a biopolymer is provided by developing a fluorescent colorant compound on the basis of a principle that is universally applicable to existing fluorescent colorants having various fluorescent properties, the fluorescent colorant compound being a compound which forms a complex with a biopolymer through a metal ion having no oxidation-reduction reactivity, is capable of selectively luminescing only when associating with the biopolymer, and does not generate nonspecific background fluorescence even without conducting cleaning. The marker comprises a first compound comprising a group of atoms P that sends out a signal and a group of atoms X and a second compound comprising a group of atoms Q that absorbs the signal and a group of atoms Y, wherein the group of atoms X is a specific bonding partner of the group of atoms Y and, when the group of atoms X connects with the group of atoms Y through a noncovalent bond to associate the first compound with the second compound, then the group of atoms Q absorbs the signal sent by the group of atoms P. Also provided is a selective marker for a protein, the marker comprising HisZiFiT as the first compound and BHQ2-His6 or DABCYL-His6 as the second compound or comprising TMR-triNTA as the first compound and DABCYL-His6 as the second compound.