发明名称 |
SEQUENCE-SPECIFIC NUCLEIC ACID PURIFICATION METHOD MANNER |
摘要 |
Disclosed is a method for the purification and collection of a nucleic acid comprising a specific nucleotide sequence, which can be carried out within an extremely short period and can achieve both high sequence-specificity and a high collection rate. Specifically disclosed is a method for the purification of a target nucleic acid comprising a specific nucleotide sequence and contained in a nucleic acid mixture. The method comprises the steps of: hybridizing a photo-ligating nucleic acid having a group represented by formula (I) as abase moiety with the target nucleic acid to form a hybrid; irradiating the hybrid of the photo-ligating nucleic acid and the target nucleic acid with light to cause the photo-ligation of the hybrid; removing any un-photo-ligated nucleic acid by washing; and irradiating the hybrid of the photo-ligating nucleic acid and the target nucleic acid with light to cause the photo-cleavage of the hybrid.
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申请公布号 |
US2011034683(A1) |
申请公布日期 |
2011.02.10 |
申请号 |
US20090937471 |
申请日期 |
2009.04.16 |
申请人 |
JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY;JAPAN SCIENCE AND TECHNOLOGY AGENCY |
发明人 |
FUJIMOTO KENZO;YOSHIMURA YOSHINAGA |
分类号 |
C07H1/06;C07H21/00;C07K2/00 |
主分类号 |
C07H1/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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