MASS PROPAGATION METHOD OF ADVENTITIOUS ROOT PHYLLANTHUS URINARIA

摘要

PURPOSE: A method for producing a large amount of adventitious roots of Phyllanthus Urinaria is provided to prevent callus formation and to normally form entire plant shape and root part. CONSTITUTION: A method for producing a large amount of adventitious roots of Phyllanthus Urinaria comprises: a step of sterilizing seeds of Phyllanthus urinaria; a step of placing on 1/3 MS medium to induce germination of Phyllanthus urinaria seeds; a step of placing immature branch of Phyllanthus urinaria in MS medium containing IBA(indole-3-butyric acid) for inducing of first adventitious root; a step of culturing the first adventitious root on MS liquid medium containing IBA and sucrose to induce second adventitious root; and a step of culturing the second adventitious root.