DEVELOPMENT OF KOREAN NATIVE PIGS SPECIFIC MOLECULAR MARKER AND METHOD FOR DETECTING KOREAN NATIVE PIGS USING THE MARKER

摘要

PURPOSE: A development for Korean traditional pig molecular marker and a method for determining Korean traditional pig using the same are provided. CONSTITUTION: A method for distinguishing Korean traditional pig breed and improved pig breed comprises: a step of amplifying KIT gene of the Korean traditional pig breed and improved pig breed(Landrace and Large white) using a primer of sequence numbers 5 and 6; a step of performing PCR-RFLP(restriction fragment length polymorphism) including ACC II restriction enzyme treatment. The primers has a forward primer of sequence number 5 having base sequence, GTAAGGCCCAGATGTTCTTCTT; and reverse primer of sequence number 6 having base sequence, CAACCGGACCTACTGAAATGAC.