METHOD FOR CONTROLLING PLASMID COPY NUMBER IN E. COLI

摘要

Host vector system and methods for plasmid DNA and recombinant protein production. The system allows copy number control of a CoIE1 plasmid in E. coli by an RNA molecule that is transcribed from the host's genome and that interacts with plasmid-transcribed RNAI or RNAII. The system can be extended to combine PCN control and antibiotic-free selection.