发明名称 |
Method of forming dendritic cells from embryonic stem cells |
摘要 |
This invention relates to the culture of dendritic cells from human embryonic stem (ES) cells. Human ES cells are first cultured into hematopoietic cells by co-culture with stromal cells. The cells now differentiated into the hematopoietic lineage are then cultured with GM-CSF to create a culture of myeloid precursor cells. Culture of the myeloid precursor cells with the cytokines GM-CSF and IL-4 causes functional dendritic cells to be generated. The dendritic cells have a unique phenotype, as indicated by their combination of cell surface markers. |
申请公布号 |
AU2006252576(B2) |
申请公布日期 |
2011.01.20 |
申请号 |
AU20060252576 |
申请日期 |
2006.05.31 |
申请人 |
WISCONSIN ALUMNI RESEARCH FOUNDATION |
发明人 |
JAMES A. THOMSON;IGOR I. SLUKVIN;MARYNA E. GUMENYUK;MAKSYM A. VODYANYK |
分类号 |
C12N5/073;C12N5/0784 |
主分类号 |
C12N5/073 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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