发明名称 |
METHOD FOR EXPRESSION OF SMALL ANTIVIRAL RNA MOLECULES WITH REDUCED CYTOTOXICITY WITHIN A CELL |
摘要 |
<p>In one aspect, the invention provides methods and compositions for the expression of small RNA molecules within a cell using a retroviral vector (FIG. 1A). Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell. In a further aspect, the invention provides methods for producing siRNA encoding lentivirus where the siRNA activity may interfere with the lentiviral life cycle. In yet a further aspect, the invention provides methods for expression of a small RNA molecule within a cell, such as an siRNA capable of downregulating CCR5, wherein expression of the small RNA molecule is relatively non-cytotoxic to the cell. The invention also includes small RNA molecules, such as an siRNA capable of downregulating CCR5, that are relatively non-cytotoxic to cells.</p> |
申请公布号 |
EP2115164(A4) |
申请公布日期 |
2011.01.19 |
申请号 |
EP20080731694 |
申请日期 |
2008.03.07 |
申请人 |
CALIFORNIA INSTITUTE OF TECHNOLOGY;THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
LOIS-CABALLE, CARLOS;BALTIMORE, DAVID;QIN, XIAO-FENG;CHEN, IRVIN, S.Y.;AN, DONG, SUNG |
分类号 |
C12N15/09;A61K31/7088;A61K48/00;A61P31/18;C07H21/02;C07H21/04;C12N15/113;C12N15/63;C12N15/867 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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