Fermentation of moderately thermophilic Bacilli on sucrose

摘要

The present invention discloses a method for the construction of a moderately thermophilic Bacillus strain capable of utilising sucrose as a carbon source comprising the transformation of a parent moderately thermophilic Bacillus strain not capable of utilising sucrose as a carbon source with a polynucleotide comprising a DNA sequence that encodes a polypeptide having sucrose-specific phosphotransferase activity and having i) an amino acid sequence of SEQ ID NO:1 or ii) an amino acid sequence with an identity of at least 70% to the sequence of SEQ ID NO:1 and/or comprising a DNA sequence that encodes a polypeptide having sucrose-6-phosphate hydrolase activity and having iii) an amino acid sequence of SEQ ID NO:2 or iv) an amino acid sequence with an identity of at least 70% to the sequence of SEQ ID NO:2. The present invention further discloses a method for the production of a compound of interest comprising the cultivation of the transformed moderately thermophilic Bacillus strain on a sucrose-containing carbon source. Finally, novel polypeptides and polynycleotides enabling sucrose utilization are disclosed.