| 摘要 |
<p>The invention concerns mycobacteria-specific oligonucleotides sequences whose physico-chemical characteristics will be adequately variable to allow their further conjugation to quantum dots (QDs) in a way that will secure optimum performance (for example in systems based on Quantum Dots (QDs). To this goal we have constructed a set of oligonucleotide probes that bind selectively to highly conserved regions of the mycobacterial genome and can be conjugated cadmium selenite QDs, aiming to the simultaneous detection of several mycobacterial DNA targets without molecular amplification. The use of quantum dots (QDs)-universal-probes allows rapid and direct detection collectively of the main mycobacterial pathogens [ Mycobacterium tuberculosis complex (MTC), M. avium complex (MAV) and M. avium subsp paratuberculosis (MAP)] in clinical samples, in a way that is highly specific, very easy to perform, and requires minimum infrastructure and expertise.</p> |
