Whole-cell catalytic system comprising a hydantoinase, a racemase and a carbamoylase

摘要

The present invention relates to a whole cell catalytic system for the preparation of an enantiomerically enriched a-amino acid from a corresponding hydantoin wherein hydantoinase, L-carbamoylase and hydantoin racemase are coexpressed in a recombinant micro-organism wherein the genes coding for these three enzymes are located on a single replicon. The present invention further relates to the use of such a whole cell catalytic system according in the preparation of an enantiomerically enriched L-±-amino acid from a corresponding hydantoin.