Compartmentalized device for cell culture, cell processing, and sample dialysis

摘要

A method of culturing cells in a compartmentalized roller bottle including a basal medium compartment and a cell culture compartment residing within a roller bottle housing, an access port to said cell culture compartment, and an access port to said basal medium compartment, said cell culture compartment being bounded by at least a portion of said roller bottle housing, at least a portion of said basal medium compartment being comprised of a semi-permeable membrane, the method comprising
a. adding a cell culture medium and adding cells into said cell culture compartment;
b. adding a basal medium into said basal medium compartment;
c. placing said compartmentalized roller bottle onto a roller rack and allowing said roller bottle housing to rotate upon said roller rack wherein at least a portion of said semi-permeable membrane resides directly below at least a portion of said basal medium when said compartmentalized roller bottle is rolling; and
d. said cell culture medium making at least periodic contact with said semi-permeable membrane when said compartmentalized roller bottle is rolling