| 摘要 |
The present invention relates to an improved method, reagents and kit for the detection of fecal or urine occult blood. The reagents are either supplied as solutions in small vials or incorporated into a matrix that is treated, impregnated, or imprinted with the test reagents that are capable of undergoing a chromagenic reaction. The method elucidates false positives from dietary sources, oxidants in the toilet water supply and is specific for blood. A stabilized chromogenic solution is disclosed which contains phenolphthalin in a deoxygenated, basic solution. The chromagenic solution either in a liquid or matrix form is placed into the toilet water following urination or defecation. A few moments are allowed to occur for observation of the matrix turning from colorless to a hot-pink. A hot pink color is indicative of a false positive. If no color change occurs, an oxidizing solution is added to the toilet water. If a change from colorless to a hot-pink occurs in less than thirty seconds then this test is positive and specific for occult blood in this fecal and/or urine specimen. Typically, the oxidizing solution is hydrogen peroxide. Hemoglobin or a porphyrin solution can be added to a separate matrix or otherwise be provided as a positive control. |
