DNA FRAGMENT, METHOD FOR PRODUCTING TRANSFORMANT FOR PRODUCING TRANSFORMANT FOR PROTEIN PRODUCTION AND UTILIZATION THEREOF

摘要

An expression vector is constructed by transferring recombinant tomato mosaic virus (ToMV) cDNA, in which a coat protein of ToMV having a suppressor for a virus resistant reaction has been substituted by a GFP gene, into the downstream of a promoter capable of inducing steroid hormone-dependent transcription. In a transformed tobacco BY-2 cell obtained by transferring the above expression vector into a tobacco BY-2 cell, steroid hormone-dependent transcription is induced, thereby enabling the amplification of mRNA of the GFP gene and induction of the expression of GFP.