| 摘要 |
<p>The present invention provides a method for improving a leader sequence by insertion of a pentapeptide motif comprising -X 1 -X 2 -X 3 -X 4 -X 5 - and a polypeptide comprising (i) a leader sequence, the leader sequence comprising a (a) secretion pre sequence, and (b) the following motif:
-X 1 -X 2 -X 3 -X 4 -X 5 -
where X 1 is phenylalanine, tryptophan, or tyrosine, X 2 is isoleucine, leucine, valine, alanine or methionine, X 3 is leucine, valine, alanine or methionine, X 4 is serine or threonine and X 5 is isoleucine, valine, alanine or methionine; and (ii) a desired protein heterologous to the leader sequence. A polypeptide of the invention may additionally comprise, as part of the leader sequence, a secretion pro sequence. The invention also provides a polynucleotide comprising a sequence that encodes a polypeptide of the invention and a cell, preferably a yeast cell, comprising said polynucleotide.</p> |
