发明名称 |
VERFAHREN ZUR KULTIVIERUNG DES NITRILHYDRATASE-PRODUZIERENDEN STAMMES RODOCOCCUS RHODOCHROUS M33 |
摘要 |
Method for culturing the nitrile hydratase (I)-producing strain Rhodococcus rhodochrousM33 (DSM 14230) using a medium based on 12-60 mM phosphate buffer that (a) satisfies the cells' requirement for phosphorous and (b) maintains pH 5.5-9 during culture. An independent claim is also included for a medium for the process comprising, per l, 7.9 g sodium dihydrogenphosphate dodecahydrate; 1.8 g potassium dihydrogenphosphate; 0.02-0.04 g cobalt(II) chloride hexahydrate; 1 g magnesium sulfate heptahydrate; 0-10 g maize extract; 20-90 g glucose and 4-20 g urea. |
申请公布号 |
DE502004011806(D1) |
申请公布日期 |
2010.12.02 |
申请号 |
DE20045011806T |
申请日期 |
2004.04.06 |
申请人 |
ASHLAND LICENSING AND INTELLECTUAL PROPERTY LLC |
发明人 |
LORENZ, JOSEF;WORONIN, S. P.;KOSOLIN, S. W.;SINGIRZEV, I. N.;DEBABOV, V. G.;YANENKO, A. S. |
分类号 |
C12N1/20;C12N1/21;C12N1/38;C12N9/80;C12N9/88;C12P13/02;C12P21/06 |
主分类号 |
C12N1/20 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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