PROMOTER AND PLASMID SYSTEM FOR GENETIC ENGINEERING

摘要

PROBLEM TO BE SOLVED: To solve problems remaining to be solved of how to easily and quickly clone a plurality of genes or operons while minimizing the impact of metabolic load, controlling an amount of production of a recombinant type protein to meet production needs, and enhancing the stability of transformed host cells.

SOLUTION: There are provided a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least the three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where a plurality of genetic insertions are sought.

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