发明名称 |
CSF-1R MUTANTS |
摘要 |
Provided are mutant class III receptor tyrosine kinases (RTKIII) comprising a mutation at the amino acid residue corresponding to the conserved cysteine at residue 432 (C432) or 439 (C439) of a mouse colony-stimulating factor (1) receptor (CSF-IR) precursor having the amino acid sequence of SEQ ID NO: 1, where the mutation is a replacement of the cysteine with an amino acid having an uncharged polar R group. Also provided are mutant RTKIIIs comprising a tyrosine to phenylalanine mutation. Additionally provided are extracellular domains of the above-identified mutant RTKIIIs comprising cysteine to serine mutations. Further provided are stable cell lines of macrophages lacking a native CSF-IR. Also provided are isolated nucleic acids encoding any of the above-described mutant RTKIIIs or extracellular domains. Vectors comprising those nucleic acids are also provided. Additionally provided are methods of preparing the above-identified stable cell lines. Methods of treating a mammal comprising administering the above described extracellular domain to the mammal are also provided.
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申请公布号 |
US2010298217(A1) |
申请公布日期 |
2010.11.25 |
申请号 |
US20080451680 |
申请日期 |
2008.05.22 |
申请人 |
STANLEY EVAN RICHARD;XIONG YING |
发明人 |
STANLEY EVAN RICHARD;XIONG YING |
分类号 |
A61K38/17;A61P3/04;A61P9/10;A61P25/00;A61P25/28;A61P29/00;A61P35/00;A61P37/02;C07K14/705;C12N5/02;C12N5/078;C12N9/12 |
主分类号 |
A61K38/17 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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