发明名称 |
CULTURE MEDIUM FOR EPITHELIAL STEM CELLS AND ORGANOIDS COMPRISING SAID STEM CELLS. |
摘要 |
<p>The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising said epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of said culture medium, and to crypt-villus organoids, gastric organoids and pancreatic organoids that are formed in said culture medium.</p> |
申请公布号 |
WO2010090513(A3) |
申请公布日期 |
2010.11.25 |
申请号 |
WO2010NL00017 |
申请日期 |
2010.02.03 |
申请人 |
KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN;SATO, TOSHIRO;CLEVENS, JOHANNES, CAROLUS;HUCH ORTEGA, MERITXELL |
发明人 |
SATO, TOSHIRO;CLEVENS, JOHANNES, CAROLUS;HUCH ORTEGA, MERITXELL |
分类号 |
C12N5/073 |
主分类号 |
C12N5/073 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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